Brian C. Smith, Ph.D.
Assistant Professor of Biochemistry


B.S. 2003, Chemistry University of Illinois at Urbana-Champaign

Ph.D. 2008, Organic Chemistry, Laboratory of John Denu, University of Wisconsin-Madison

Postdoc, 2009-2014, Nitric Oxide Signaling, Laboratory of Michael Marletta, University of California-Berkeley and Scripps Research Institute


Smith Lab Publications

33. Hanson QM, Carley JR, Gilbreath TJ, Smith BC, Underbakke ES. “Calmodulin-induced Conformational Control and Allostery Underlying Neuronal Nitric Oxide Synthase Activation”. Journal of Molecular Biology. 2018, 430, 935-47.

32. Fahey JM, Stancill JS, Smith BC, Girotti AW. “Antagonistic effects of nitric oxide in a glioblastoma photodynamic therapy model: mitigation by BET bromodomain inhibitor JQ1”. Journal of Biological Chemistry. 2018, 293, 5345-59.

31. Egner JM, Jensen DR, Olp MD, Kennedy NW, Volkman BF, Peterson FC, Smith BC, Hill RB. “Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein-Detected NMR Spectroscopy”. ChemBioChem. 2017, 19, 448-58. Appeared as the Cover Article

30. Olp MD, Zhu N, Smith BC. “Metabolically Derived Lysine Acylations and Neighboring Modifications Tune the Binding of the BET Bromodomains to Histone H4”. Biochemistry. 2017, 56, 5485-5495.

29. Wynia-Smith SL, Smith BC. “Nitrosothiol formation and S-nitrosation signaling through nitric oxide synthases”. Nitric Oxide. 2017, 63, 52-60.

28. Kalous KS, Wynia-Smith SL, Olp MD, Smith BC. “Mechanism of Sirt1 NAD+-dependent Deacetylase Inhibition by Cysteine S-nitrosation”. Journal of Biological Chemistry. 2016, 291, 25398-410.

27. Zhou Y, Wynia-Smith SL, Couvertier, SM, Kalous KS, Marletta MA, Smith BC*, Weerapana E. “Chemoproteomic Strategy to Quantitatively Monitor Transnitrosation Uncovers Functionally Relevant S-Nitrosation Sites on Cathepsin D and HADH2”. Cell Chemical Biology. 2016, 23, 727-37. *Co-corresponding author.

26Shteyer E and Smith BC. “Tail Tale: nNOS(del1203-1434) Predicts Global Defects in Esophagogastrointestinal Transit Reply”. Gastroenterology. 2015, 149, 261-2.

25. Shteyer E, Edvardson S, Wynia-Smith SL, Pierri CL, Zangen T, Hashavya S, Begin M, Yaacov B, Cinamon Y, Koplewitz BZ, Vromen A, Elpeleg O, Smith BC. “Truncating Mutation in the Nitric Oxide Synthase 1 Gene Is Associated with Infantile Achalasia”. Gastroenterology. 2015, 148, 533-536.


24. Bruegger JJ, Smith BC, Wynia-Smith SL, Marletta MA. “Comparative and integrative metabolomics reveal that S-nitrosation inhibits physiologically relevant metabolic enzymes”. Journal of Biological Chemistry. 2018, 293, 6282-96.

23. Rao M, Smith BC, Marletta MA. “Nitric Oxide Mediates Biofilm Formation and Symbiosis in Silicibacter sp. Strain TrichCH4B”. MBio. 2015, 6, e00206-15.

22. Campbell MG, Smith BC, Potter CS, Carragher B, Marletta MA. “The molecular architecture of mammalian nitric oxide synthases”. Proceedings of the National Academy of Sciences. 2014, 111, E3614-23.

21. Smith BC, Underbakke ES, Kulp DW, Schief WR, Marletta MA. “Nitric oxide synthase domain interfaces regulate electron transfer and calmodulin activation”. Proceedings of the National Academy of Sciences. 2013, 110, E3577-86.

20. Underbakke ES, Surmeli NB, Smith BC, Wynia-Smith SL, Marletta MA. “Nitric oxide signaling”. Comprehensive Inorganic Chemistry II. 2013, Vol. 3, 241-262.

19. Smith BC and Marletta MA. “Mechanisms of S-nitrosothiol formation and selectivity in nitric oxide signaling”. Current Opinion in Chemical Biology. 2012, 16, 498–506.

18. Smith BC, Fernhoff NB, Marletta MA. “Mechanism and Kinetics of Inducible Nitric Oxide Synthase Auto-S-Nitrosation and Inactivation”. Biochemistry. 2012, 51, 1028–40.


17. Smith BC, Anderson MA, Hoadley KA, Keck JL, Cleland WW, Denu JM. “Structural and Kinetic Isotope Effect Studies of Nicotinamidase (Pnc1) from Saccharomyces cerevisiae”. Biochemistry. 2012, 51, 243–56.

16. Kasamatsu A, Nakao M, Smith BC, Comstock LR, Ono T, Kato J, Denu JM, Moss J. “Hydrolysis of O-Acetyl-ADP-ribose Isomers by ADP-ribosylhydrolase 3”. Journal of Biological Chemistry. 2011, 286, 21110–7.

15. Hallows WC, Yu W, Smith BC, Devries MK, Ellinger JJ, Someya S, Shortreed MR, Prolla T, Markley JL, Smith LM, Zhao S, Guan KL, Denu JM. “Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction”. Molecular Cell. 2011, 41, 139–49.

14. Smith BC, Settles B, Hallows WC, Craven MW, Denu JM. “SIRT3 substrate specificity determined by peptide arrays and machine learning”. ACS Chemical Biology. 2011, 6, 146–57. Appeared as the Cover Article

13. Smith BC, Hallows WC, Denu JM. “A continuous microplate assay for sirtuins and nicotinamide-producing enzymes”. Analytical Biochemistry. 2009, 394, 101–9.

12. Hallows WC, Smith BC, Lee S, Denu JM. “Ure(k)a! Sirtuins Regulate Mitochondria”. Cell. 2009, 137, 404–6.

11. Smith BC and Denu JM. “Chemical mechanisms of histone lysine and arginine modifications”. Biochimica et Biophysica Acta. 2009, 1789, 45–57.

10. Smith BC, Hallows WC, Denu JM. “Mechanisms and molecular probes of sirtuins”. Chemistry & Biology. 2008, 15, 1002–13.

9. Smith BC and Denu JM. “Mechanism-based inhibition of Sir2 deacetylases by thioacetyl-lysine peptide”. Biochemistry. 2007, 46, 14478–86.

8. Smith BC and Denu JM. “Acetyl-lysine analog peptides as mechanistic probes of protein deacetylases”. Journal of Biological Chemistry. 2007, 282, 37256–65.

7. Garske AL, Smith BC, Denu JM. “Linking SIRT2 to Parkinson's disease”. ACS Chemical Biology. 2007, 2, 529–32.

6. Smith BC and Denu JM. “Sir2 deacetylases exhibit nucleophilic participation of acetyl-lysine in NAD+ cleavage”. Journal of the American Chemical Society. 2007, 129, 5802–3.

5. Smith BC and Denu JM. “Sirtuins caught in the act”. Structure. 2006, 14, 1207–8.

4. Smith BC and Denu JM. “Sir2 Protein Deacetylases: Evidence for Chemical Intermediates and Functions of a Conserved Histidine”. Biochemistry. 2006, 45, 272–82.

3. Grubisha O, Smith BC, Denu JM. “Small molecule regulation of Sir2 protein deacetylases”. FEBS Journal. 2005, 272, 4607–16.

2. Borra MT, Smith BC, Denu JM. “Mechanism of human SIRT1 activation by resveratrol”. Journal of Biological Chemistry. 2005, 280, 17187–95.

1. Schmidt MT, Smith BC, Jackson MD, Denu JM. “Coenzyme specificity of Sir2 protein deacetylases: implications for physiological regulation”. Journal of Biological Chemistry. 2004, 279, 40122–9.

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